Plasmid_Backbone

Part:BBa_K2100015:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-14)


pDEST_mCherry_GG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2466
    Illegal XbaI site found at 2493
    Illegal XbaI site found at 2797
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1907
    Illegal PstI site found at 2505
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2466
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1907
    Illegal PstI site found at 2505
    Illegal NotI site found at 971
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2466
    Illegal BamHI site found at 2487
    Illegal XhoI site found at 1031
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2466
    Illegal XbaI site found at 2493
    Illegal XbaI site found at 2797
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1907
    Illegal PstI site found at 2505
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2466
    Illegal XbaI site found at 2493
    Illegal XbaI site found at 2797
    Illegal SpeI site found at 2054
    Illegal PstI site found at 1907
    Illegal PstI site found at 2505
    Illegal NgoMIV site found at 3360
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2749
    Illegal BsaI site found at 4337
    Illegal BsaI.rc site found at 2092


Design Notes

DESIGN DECISIONS HERE!


Source

This is synthesized from cloning.

References